Resources for hiPSCs

What are the resources needed to get started for creating human iPSCs (induced pluripotent stem cells) and for induction of human iPSCs towards certain cell types (e.g. cardiomyocytes)?

· What are the reagents needed?

· Any good reading materials that you will suggest?

· Any video links that is good to watch?

· Others?

This paper provides very comprehensive protocol for iPSC reprogramming, including the timeline, and required reagents in detail.

Dowey SN, Huang X, Chou B-K, Ye Z, Cheng L. Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression. Nature Protocols. 2012;7:2013.

On the Allen Institute for Cell Science website, we provide several videos and .pdf files describing best methods for using hiPSCs in the lab, ranging from gene editing to cardiomyocyte differentiation and 3D image acquisition.

Resources for using hiPSCs:

PDFs for SOPs:

Videos from the lab highlighting nuanced techniques and helpful tips: